elegans in 1990 ( Harrison et al., 1990) and was the reporter gene of choice until the introduction of GFP in 1994 ( Chalfie et al., 1994). coli gene LacZ, encoding β-galactosidase, was first used for single-cell gene expression analysis in C. Finally, germline transformation techniquesĪllow for the rapid generation of transgenic animals. In addition, the relative thinness of animals (approximately 75 μm cross-section) often alleviates the need for high-powered confocal microscopy. elegans allows for microscopic analysis in vivo without animal dissection. The reporter genes green fluorescent protein (GFP Chalfie et al., 1994) and βGAL (LacZ Fire et al., 1990). elegans has distinguishing characteristics that predestine this organism for the visualization of gene expression patterns using
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